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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become noticed in several tumors, which leads to chromosome instability, and therefore could lead to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a essential position in advertising and marketing mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are explained. Two more splice variants had been noted but comprehensive nucleotide sequence data is not nevertheless readily available.
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Blocking peptides are peptides that bind particularly to the focus on antibody and block antibody binding. These peptide typically consists of the epitope regarded via the antibody.
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This guidebook outlines more attainable optimization of cellular and immunoassay parameters to be sure the absolute best final results are...
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This manual outlines even more feasible optimization of cellular and immunoassay parameters to ensure the absolute best outcomes are...
Antibodies bound to the blocking peptide no longer bind to your epitope about the target protein. By comparing the staining from your blocked antibody compared to the antibody by yourself, you can see which staining is specific.
Goal Data Cyclin E belongs into the really conserved cyclin family members, whose members are characterized by a spectacular periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Distinct cyclins exhibit unique expression and degradation patterns which add into the temporal coordination of every mitotic function. Cyclin E types a complex with and capabilities for a regulatory subunit of CDK2, whose exercise is necessary for mobile cycle G1/S changeover. This protein accumulates at the G1-S section boundary which is degraded as cells development by S phase.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation check here by both equally antibodies...
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